Synthesis of silver nanoparticles by using leaflet extract of Nephrolepis exaltata L. and evaluation of antibacterial activity against human and plant pathogenic bacteria
Abstract
Gajanan Bhor1, Shilpa Maskare2, Sujata Hinge3, Laxmi Singh4 and Abasaheb Nalwade5*
Silver nanoparticles were synthesized by using leaflet extract of a fern
Nephrolepis exaltata (L.) which reduces silver ions to silver
nanoparticles. Synthesized nanoparticles were confirmed by UV-Visible
spectrophotometer and UV-Visible absorption spectra of the reaction
mixture showed λmax at 420 nm. The average particles size was
confirmed by XRD peaks was 24.76 nm. SEM image showed aggregates
of spherical silver nanoparticles. Antibacterial efficiency of silver
nanoparticles was evaluated by disc diffusion assay method. Silver
nanoparticles exhibited antibacterial activity against Klebsiella
pneumonia NCIM 2719, Proteus morgani NCIM 2719, Corynebacterium
diptheriae, Pseudomonas testesteroniae NCIM 5098, Bacillus subtilis
NCIM 2063, Escherichia coli, and Xanthomonas axonopodis pv. punicae.
This cost-effective, eco-friendly and easily scald up biosynthesis method
of silver nanoparticles using leaflet extract of a fern Nephrolepis
exaltata (L.) will be compatible for pharmaceutical and medical
applications.
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