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Method Development and Validation of Milnacipran by using RP-HPLC Method | Abstract

Asian Journal of Pharmaceutical Technology and Innovation (ajpti)

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Method Development and Validation of Milnacipran by using RP-HPLC Method

Abstract

Palleppagari Sridhar*, K. Venkateswar Rao, C. Radhika, A. Sujitha, V. Uma Maheswara Rao

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Milnacipran, in its pure form as well as in capsule dosage form. Chromatography was carried out on a phenomanix C18 (4.6 x 250mm, 5µm) column using a mixture of Acetonitrile: Triethylamine buffer pH 4.5(65:35% v/v)as the mobile phase at a flow rate of 0.8ml/min, the detection was carried out at 215 nm. The retention time of the Milnacipran was 2.16 min respectively. The method produce linear responses in the concentration range 5-25 mg/ml of Milnacipran. The method precision for the determination of assay was below 2.0% RSD. The method is useful in the analysis of bulk and pharmaceutical formulations of Millancipran.

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