Isolation, Characterization and Biological Activities of 1-(5-Hydroxypentyloxy) Propan-2- One from Ethanolic Rhizome Extract of Kaempferia galanga
Abstract
Narasinga Rao V1, Dowluru Svgk Kaladhar2,* Sreenu Barla3
Plants contain several phytocompounds that shows interaction with various proteins involved in diseases. An isolation and characterization of a phytocompound from ethanolic rhizome extract of K. galanga was conducted. Characterization of a novel compound was done using UV, proton, 13C, mass and IR spectra’s. K. galanga contains a novel phytocompound 1-(5- hydroxypentyloxy) propan-2-one identified based on spectra’s. Various biological activities like antioxidant, antimicrobial, protease inhibition and anti-proliferation activities were tested for 1-(5-hydroxypentyloxy) propan-2-one. The antioxidant activities with IC50 for 1-(5-hydroxypentyloxy) propan-2-one was shown 44.5 μg/ml and IC50 for standard (Ascorbic acid) was shown as 24.5μg/ml. The 1-(5-hydroxypentyloxy) propan-2-one were shown good antifungal activities (9 to 12 mm) along with antibacterial activities (10 to 12 mm). The standard antibiotic like Tetracyclin shown zone of inhibition as 10 to 14mm for tested bacteria and for fungi standard as Fluconazole shown zone of inhibition from 13 to 16 mm. The protease inhibition studies for 1-(5-hydroxypentyloxy) propan-2-one of K. galanga have shown the good inhibition activity with enzymes like protease K and trypsin. The dose response of 1-(5-hydroxypentyloxy) propan-2- one from K. galanga on MCF-7 (Breast Cancer) cell line shown very less activity 130 μgm/ml when compared with the standard tamoxifen 12.5 μgm/ml. Hence the experimentations concluded that a phytocompound, 1-(5-hydroxypentyloxy) propan-2-one from ethanolic rhizome extract of K. galanga shown biological activities.
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