A Review On Ultra Performance Liquid Chromatography (UPLC)
Abstract
Anagha S. Patil*
In recent years, significant technological advances have been made in particle chemistry performance, system optimization, detector design, and data processing and control. When brought together, the individual achievements in each discipline have created a stepfunction improvement in chromatographic performance. Ultra performance liquid chromatography (UPLC) is a new category of analytical separation science that retains the practicality and principles of HPLC, while increasing the overall interlaced attributes of speed, sensitivity and resolution. UPLC can be regarded as a new direction for liquid chromatography. UPLC improves in three areas: “speed, resolution and sensitivity”. In this system uses fine particles (less than 2.5 μm) so decrease the length of column, saves time and reduces solvent consumption.
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