• Development and Validation of New RP-HPLC Method for the Estimation of Dasatinib in Pharmaceutical Dosage Forms

      A simple, precise, accurate RP-HPLC method was developed and validated for the estimation of Dasatinib in pharmaceutical dosage forms. An Cosmicsil BDS C18 column (150 mm x 4.6 mm), 5 µ particle size was used as stationary phase with mobile phase consisting of Phosphate buffer: a mixture of acetonitrile and methanol in the ratio of 50:50, v/v. The flow rate was maintained at 1 mL/min and effluents were monitored at 315 nm. The retention time was 6.4675 min. The linearity of the method was observed in the concentration range of 20-60 µg/mL with correlation coefficient of 0.999. The percentage assay of Dasatinib was 100.10%. The method was validated for its accuracy, precision and system suitability. The results obtained in the study were within the limits of ICH guidelines and hence this method can be used for the estimation of Dasatinib in pharmaceutical dosage forms. Download
    • Development and Validation of UV Spectrophotometric Method and RP – HPLC Method for Simultaneous Estimation of Teneligliptin and Pioglitazone In Synthetic Mixture

      The present Article portrays simple, sensitive, accurate, precise and cost effective First order derivative Spectrophotometric method and RP-HPLC method for the simultaneous estimation of Teneligliptin and Pioglitazone in Synthetic Mixture. In The first order derivative method absorption at 228.5 nm (zero crossing point for Pioglitazone) was used for Teneligliptin and 269.2 nm (zero crossing point for Teneligliptin) was used for Pioglitazone. The linearity was taken in the concentration range of 2-10 µg/ml for Teneligliptin and 3-15 µg/ml for Pioglitazone with correlation coefficient (R2) 0.995 and 0.997, respectively. For The RP-HPLC method linearity was taken in the concentration range of 1- 5 µg/ml for Teneligliptin and 1.5-7.5 µg/ml for Pioglitazone with correlation coefficient (R2) 0.998 and 0.996, respectively. Proposed technique has been validated as per ICH guideline and successfully applied to the simultaneous estimation of Teneligliptin and Pioglitazone in their Synthetic Mixture. The results of analysis have been validated statistically and by recovery studies. Download
    • Dietary Requirements for Parkinson’s Disease-A Review

      Parkinson’s disease is a gradually growing abnormality of the CNS (central nervous system). There is no definite causation and cure for this disease is known till date. It is a chronic disease and it mainly affects that portion, which is associated with the movement. There is no known remedy till date that can cure Parkinson’s disease (PD). The changing diet protocols for Parkinson’s disease works by restoring the normal biochemical level of the human body and also to promote good and healthy lifestyle. The most promising approach appears to be the use of antioxidants to slow the oxidation and damage to the substantia nigra. The most excellent diet guidance for most people with Parkinson's to have the right balance of nutrients from different food groups. That can be achieved by eating a well balanced diet, which will provide adequate levels of vitamins and minerals. It’s possible that additional nutritional approaches may be found in the future. Download
    • Development and Validation of New RP-HPLC Method for the Estimation of Dasatinib in Pharmaceutical Dosage Forms

      A simple, precise, accurate RP-HPLC method was developed and validated for the estimation of Dasatinib in pharmaceutical dosage forms. An Cosmicsil BDS C18 column (150 mm x 4.6 mm), 5 µ particle size was used as stationary phase with mobile phase consisting of Phosphate buffer: a mixture of acetonitrile and methanol in the ratio of 50:50, v/v. The flow rate was maintained at 1 mL/min and effluents were monitored at 315 nm. The retention time was 6.4675 min. The linearity of the method was observed in the concentration range of 20-60 µg/mL with correlation coefficient of 0.999. The percentage assay of Dasatinib was 100.10%. The method was validated for its accuracy, precision and system suitability. The results obtained in the study were within the limits of ICH guidelines and hence this method can be used for the estimation of Dasatinib in pharmaceutical dosage forms. Download
    • Dietary Requirements for Parkinson’s Disease-A Review

      Parkinson’s disease is a gradually growing abnormality of the CNS (central nervous system). There is no definite causation and cure for this disease is known till date. It is a chronic disease and it mainly affects that portion, which is associated with the movement. There is no known remedy till date that can cure Parkinson’s disease (PD). The changing diet protocols for Parkinson’s disease works by restoring the normal biochemical level of the human body and also to promote good and healthy lifestyle. The most promising approach appears to be the use of antioxidants to slow the oxidation and damage to the substantia nigra. The most excellent diet guidance for most people with Parkinson's to have the right balance of nutrients from different food groups. That can be achieved by eating a well balanced diet, which will provide adequate levels of vitamins and minerals. It’s possible that additional nutritional approaches may be found in the future. Download
    • Development and Validation of UV Spectrophotometric Method and RP – HPLC Method for Simultaneous Estimation of Teneligliptin and Pioglitazone In Synthetic Mixture

      The present Article portrays simple, sensitive, accurate, precise and cost effective First order derivative Spectrophotometric method and RP-HPLC method for the simultaneous estimation of Teneligliptin and Pioglitazone in Synthetic Mixture. In The first order derivative method absorption at 228.5 nm (zero crossing point for Pioglitazone) was used for Teneligliptin and 269.2 nm (zero crossing point for Teneligliptin) was used for Pioglitazone. The linearity was taken in the concentration range of 2-10 µg/ml for Teneligliptin and 3-15 µg/ml for Pioglitazone with correlation coefficient (R2) 0.995 and 0.997, respectively. For The RP-HPLC method linearity was taken in the concentration range of 1- 5 µg/ml for Teneligliptin and 1.5-7.5 µg/ml for Pioglitazone with correlation coefficient (R2) 0.998 and 0.996, respectively. Proposed technique has been validated as per ICH guideline and successfully applied to the simultaneous estimation of Teneligliptin and Pioglitazone in their Synthetic Mixture. The results of analysis have been validated statistically and by recovery studies. Download
    • Drug Utilization Evaluation For Postoperative Patients In Obstetrics and Gynaecology Department In A Tertiary Care Teaching Hospital

      A growing number of pharmaceutical products are available in the world market and there has been an increase both in the consumption of the drugs and in expenditure on them. The main aim of the study was to analyze drug use pattern in a post-operative patients in obstetrics and gynaecology ward and to assess the prescribing indicators (WHO: Core Drug Use Indicators). A prospective, concurrent and retrospective observational study was conducted over a period of six months, after getting approval by the Institutional Ethics committee. A well designed patient data collection proforma for collecting the required data from post-operative patients by using chart review method in Obstetrics and Gynaecology ward of a tertiary care teaching hospital. The data was analyzed using descriptive analysis with the help of SPSS software. The results shown are a total of 582 cases were analyzed during the following study period. Out of 582 patients, 559 (96%) were prescribed with parenteral Cefotaxime and Metronidazole. Post-operative pain was managed with Tramadol (87%). All patients were prescribed with an antibacterial agent and analgesic (100%). The percentage of encounters with an injection prescribed was 100% in our study. Out of 19 different drugs prescribed, 16 (84.21%) were from the Essential Medicines WHO Model List (2015) and 10 (52.63%) were prescribed by their generic name. Accordingly, the study can be concluded as it provides valuable insight about the overall pattern of drug used in postoperative patients. The study is useful in decreasing the irrational prescription, which helps to decrease the morbidity and health care burden in the society. Download
    • Development and Validation RP-HPLC Method for Simultaneous Estimation of Telmisartan and Nifedipine In Synthetic Mixture

      A simple, specific and accurate Reverse Phase High Performance Liquid Chromatography Method was developed for the simultaneous determination of Telmisartan and Nifedipine in Synthetic Mixture. The using Phenomenex Luna C18 (250 mm x 4.6 mm, 5 μm) column in Isocratic mode, with Mobile Phase containing ACN: Water: Methanol in the ratio of (10:20:70 v/v/v) pH 3.8 adjusted with Orthophosphoric acid at detection wavelength 234 nm with flow rate is 1 ml/min and run time is 15 min. the average retention time was found to b 2.563 min and 4.403 min for TEL and NIFE respectively. The calibration was linear in concentration range of 4-20 𝜇g/mL for TEL and 2-10 𝜇g/mL for NIFE. The low RSD (< 2%) Value indicates that the method is precise. The recoveries for TEL and NIFE were found to be in the range of 99-100%. The proposed method was Validated and successfully applied for the estimation of Telmisartan and Nifedipine in Synthetic Mixture. Download
    • Development and Characterization of Mucoadhesive HBsAg PLGA Microsphere for Nasal Vaccine Delivery

      The purpose of the study was to evaluate the mucoadhesive property of Hepatitis B surface antigen (HBsAg) loaded surface modified poly(lactic-co-glycolic acid) (PLGA) microspheres for nasal vaccine delivery. The surface modification was carried out using coating material such as chitosan, Trimethyl chitosan and N-trimethyl chitosan and N-carboxymethyl chitosan (TMC-CMC). The developed formulations were characterized for surface morphology, particle size, zeta potential, entrapment efficiency, structural integrity, In vitro mucoadhesion study and mucin adsorption ability. PLGA microspheres without surface modification demonstrated negative zeta potential, whereas Chitosan and its derivatives coated microspheres showed higher positive zeta potential. Results indicated that combination of TMC-CMC coated microspheres demonstrated substantially higher mucin adsorption and longer time of mucoadhesion when compared to chitosan and TMC coated microspheres and uncoated PLGA microspheres. Both uncoated and coated PLGA microspheres showed initial burst release followed by prolonged release pattern. The immuno-adjuvant ability of various formulations was determined on the basis of specific antibody titer observed in serum and secretions of mice. In vivo immunogenicity studies showed increased anti-HBsAg titer with TCC-CMC coated PLGA microspheres as compared to other coated and uncoated PLGA microspheres. To conclude, TCC-CMC coated PLGA microspheres could be a promising carrier targeted delivery for HBsAg in nasal mucosa. Download
    • Development And In vitro Evaluation of PLGA Microsphere of Docetaxel

      Breast-targeting docetaxel loaded (polylactic-co-glycolic) acid microsphere were prepared by a solvent evaporation method. The uniform design was used to optimize the technology of preparation, the appreance and size distribution were examined by scanning electron microscope, and the aspect such as in vitro release characteristics, stability drug loading efficiency. The experimental result show that the microspheres were globular in appearance and disperse well the average particle size decrease form is 24.49±0.38.49 micro-meter to 4.99+ 0.11micrometer. The drug loading efficiency decrease75.01±1.2% to81.2±1.9% respectively. The in vitro release behaviour could be expressed by the drug release rate decrese from 90% to 84% with increasing amount of PLGA during preparation. The drug release rate increase from 81% to 98% with the increase in stirring rate from 200to800 RPM. Stability studies were carried out with selected formulation period of 45-Daysmicrospheres was found to increase slightly. Download
    • Development and Validation RP-HPLC Method for Simultaneous Estimation of Valsartan and Nifedipine in Synthetic Mixture

      A simple, specific and accurate Reverse Phase High Performance Liquid Chromatography Method was developed for the simultaneous determination of Valsartan and Nifedipine in Synthetic Mixture. The using Phenomenex Luna C18 (250 mm x 4.6 mm, 5 μm) column in Isocratic mode, with Mobile Phase containing Methanol: Water: ACN (pH 3.7 adjusted with 10% Ortho Phosphoric Acid) (70:25:05 %v/v/v). The Flow Rate was 1 ml/min and effluents were monitored at 233 nm. The Retention Time of Valsartan and Nifedipine were found to be 8.003 min and 5.290 min respectively. The Linearity for Valsartan and Nifedipine were found to be 4-20 µg/ml and 1.5-7.5 µg/ml respectively. The Recoveries of Valsartan and Nifedipine were found to be 99.50% – 100.15% and 99.33% – 100.40% respectively. The proposed method was validated and successfully applied for the estimation of Valsartan and Nifedipine in Synthetic Mixture. Download
    • Development and Validation RP-HPLC Method For Simultaneous Estimation of Cefuroxime Axetil and Linezolid In Combined Dosage Form

      A simple, specific and accurate Reverse Phase High Performance Liquid Chromatography Method was developed for the simultaneous determination of Cefuroxime Axetil and Linezolid in combined dosage form. The using Kromasil C-8 (250×4.6 mm, 5 μm) column in Isocratic mode, with Mobile Phase Acetonitrile : Phosphate buffer, pH=4.0 (60:40 % v/v) (pH 4.0 adjusted with Orthophosphoric acid). The Flow Rate was 0.9 ml/min and effluents were monitored at 268 nm. The Retention Time of were found to be Linezolid and Cefuroxime Axetil 6.860 min and 8.840 min respectively. The Linearity for Cefuroxime Axetil and Linezolid were found to be 2.5-12.5 µg/ml and 3-15 µg/ml respectively. The Recoveries of Cefuroxime Axetil and Linezolid were found to be 101.66 – 101.90% and 98.14 - 101.15 % respectively. The proposed method was validated and successfully applied for the estimation of Cefuroxime Axetil and Linezolid in combined dosage form. Download
    • Development and Validation of UV Spectrometric and HPLC Method for Estimation of Escitalopram Oxalate and Flupentixol Dihydrochloride in Combined Dosage Form

      Reverse phase High-performance liquid chromatographic (HPLC) and UV spectrophotometric methods were developed and validated for the quantitative determination of Escitalopram oxalate and Flupentixol dihydrochloride in combined dosage form. Different analytical performance parameters such as linearity, precision, accuracy, specificity, limit of detection (LOD) and limit of quantification (LOQ) were determined according to ICH Q2R1 guidelines. The RP-HPLC method was developed by the isocratic technique on a column of Kromasil C8 (250×4.6 mm, 5 μm). The retention time for ESC and Flu was 3.6 min and 5.8 min respectively. The UV spectrophotometric determinations were performed at the zero crossing point (ZCP) of ESP was found to be 238 nm and ZCP of Flu was found to be 229 nm. The linearity of the calibration curves for each analyte in the desired concentration range was good (r2 > 0.999) by both the HPLC and UV methods. The method showed good reproducibility and recovery with percent relative standard deviation less than 2%. Moreover, UV spectroscopy can be a cheap, reliable and less time consuming alternative for chromatographic analysis. The proposed methods are highly sensitive, precise and accurate and hence successfully applied for determining the assay of a Combined dosage form. Download
    • Development of a Thin Layer Chromatography Method for the Estimation of Quinine and Ciprofloxacin

      A new, rapid, and specific thin layer chromatographic method was developed for the estimation of quinine and ciprofloxacin. Separation was achieved in chromatographic tank by using adsorbent material as silica gel which was applied on glass plate and mobile phase as acetonitrile: methanol: 0.1% triethylamine in water: (1:6:3 v/v/v) was found to resolve quinine and ciprofloxacin. Detection was achieved by using iodine flakes. The selected chromatographic conditions effectively separated quinine and ciprofloxacin with the distance travelled by quinine and ciprofloxacin 2.6 cm and 1.9 cm with the distance travelled by solvent 5 cm. This gives R f value 0.52 and 0.38 for quinine and ciprofloxacin respectively. The developed method was completely novel, reproducible and specific. Download
    • Development and Validation of a RP- HPLC Method for Quantitation of Oxcarbazepine in Tablet Dosage Form

      A novel, accurate and precise HPLC method for determination of oxcarbazepine has been developed and validated. Separation was achieved on X Terra C18 column (50 x 4.6 mm internal diameter and 5μm particle size) using Ammonium Bicarbonate and Acetonitrile as a mobile phase at a flow rate of 1.0ml/min and photo diode array detection at 214nm. The developed method was applied for quantitative determination of above drugs in tablet dosage forms and the method was validated with respect to specificity, precision, linearity, accuracy, system suitability, robustness and solution stability. The method was linear over the range of 50-300ug/ml for Oxcarbazepine respectively. The mean recovery was found to be in the range of 99.01-99.28%. The percentage of relative standard deviation was found to be less than critical value. The method was found to be accurate, precise and selective for simultaneous estimation of Oxcarbazepine in tablets. Download
    • Development and Evaluation of Niosomal Formulation of Famciclovir

      Niosomes are one of the best and most effective among these carriers system. Because of the presence of hydrophilic, lipophilic and amphiphilic moieties in the structure, these niosomes can accommodate various drug molecules with a wide range of solubility. So, these may act as a depot, releasing the drug in a defined manner. Rationale behind the present study is to improve the oral bioavailability of Famciclovir by preparing niosomes. Encapsulation of Famciclovir in lipophilic vesicular structure may be expected to enhance the dissolution, oral absorption and prolong the existence of the drug in the systemic circulation. The niosomal dispersions were formulated using various combinations of cholesterol and spans. The formulations were evaluated in-vitro and in-vivo and compared with the marketed preparation of Famciclovir. Download
    • Development and Evaluation of Oral Floatable In situ Gel Containing Ranolazine

      The aim of the present study is to develop and evaluate stomach specific oral floatable in situ gel of Ranolazine using pH triggered mechanism. The in situ gel is developed using gellan gum as gelling agent and HPMC K 100M as drug release retarding polymer. Calcium chloride is added as source of Ca2+ ion required for gelation. The different batches of gels were prepared and in vitro release study for the same is carried out by USP dissolution test apparatus using 900 ml of 0.1N HCl, at temperature at 37 °C. Developed in situ gelling solutions were evaluated for pH measurement, viscosity determination, in vitro gelling studies, in vitro buoyancy studies, drug content, in vitro release study and stability studies were done according to ICH guidelines. FTIR study revealed no interaction between drug and selected polymer. pH of gels were neutral in nature. Viscosity of F8 (optimized formulation) was in acceptable range and possess enough gelling capacity. Viscosity of solutions and gel strength were increased with increase in the concentration of polymers. The F8 showed an increased floating time upto 14 hrs and drug content 92±0.23%. The in vitro release data revealed that 91.23 % of drug released over the period of 12 hrs. FT-IR and DSC studies revealed that there was no significant drug-excipient interaction and degradation. The drug release was sustained as the polymer concentration increased in the formulae. Stability study showed no remarkable changes in drug content, viscosity and other evaluation parameters. The results suggested that developed floating in situ gel could perform better than conventional dosage forms leading to improved efficacy and better patient compliance. Download
    • Development and Characterization of Mucoadhesive Drug Delivery System of Tizanidine Hydrochloride

      The buccal region offers an attractive route of administration for systemic drug delivery. The mucosa has a rich blood supply and provides rapid absorption for drugs than oral route Tizanidine hydrochlorideis a non-selective, α-2 adrenergic agonist receptor. Tizanidine hydrochloride undergoes first-pass hepatic metabolism by oral route and exhibits only 40% bioavailability. Because of poor bioavailability of Tizanidine hydrochloride by oral route, there is a need to increase its bioavailability by formulating it into buccal dosage forms. Hence, Tizanidine hydrochloride is a suitable drug for buccal dosage forms and may provide a better therapeutic profile than oral route. A number of buccal mucoadhesive patches of Tizanidine hydrochloride were prepared by solvent casting method using HPMC, Hydroxy ethyl cellulose, Carboxy methyl cellulose sodium, Carbopol and polyvinyl pyrrolidone as polymer. Propylene glycol was used as plasticizers, while the solvent chosen was water. The films were uniform and translucent. These were having good strength and visually smooth surfaced. The films were evaluated on the basis of their release characteristics, swelling Index, surface pH, folding endurance, film thickness, weight uniformity, drug content uniformity, Bursting Strength. In-vitro release studies were conducted for Tizanidine hydrochloride loaded patches in phosphate buffer, pH 6.8. Patches exhibited drug release in the range of 75.79% to 93.98% in 7th hours. The best formulation (F3) exhibit in vitro drug releases 93.17±1.56 %. This may improve the bioavailability of the Tizanidine hydrochlorideis a non-selective, α-2 adrenergic agonist receptor. Download
    • Development and Validation of Spectrophotometric Method for Analysis of Topiramate

      Selective, inexpensive and validated method was developed and optimized for determination of antiepileptic drug, Topiramate in drug substance and drug product as well as in the presence of pyridine. The method is based on the reaction of the primary amino group of Topiramate with Ninhydrin reagent in ethanolic medium in the presence of 10% pyridine solution. The colored product was measured at 568 nm. The linearity range was found to be 50 – 300 μg mL-1 with mean recovery 98-102%. All variables affecting the reaction conditions were thoroughly studied. The results were found to agree statistically, the method was validated according to the ICH guidelines. The proposed method is practical and valuable for their in quality control laboratories for analysis of Topiramate. Download
    • Development of Controlled Drug Delivery System for a Traditional Medicine and Study of Its Antimicrobial Property

      Development of a novel drug delivery system to provide sustainable and controlled release of a traditional Ayurvedic medicine, Makaradhwaja (enhances the power of immunity and prevents inflammatory and degenerative changes) has been attempted by using the process of encapsulation into nano porous silica gel matrix. Following entrapment of Makaradhwaja, the synthesized gels have been subjected to characterization by FTIR, HRTEM and UV-visible spectroscopic analysis. FTIR and HRTEM studies had been done to authenticate the presence of drug molecule into the gel matrix. In FTIR spectrum the broad peaks at 3474 cm-1and 1637 cm-1 are detected due to the presence of Makaradhwaja into silica gel. The entrapment has also been proved by the HRTEM image. Release kinetics in a Simulated Body Fluid (SBF) has been subsequently observed and the UV-visible spectroscopy reveals up to 50% release of drug molecule in the Simulated Body Fluid (SBF) in 240 hours. The significant antimicrobial activity of the formulation had been observed against three pathogenic bacterial strains i.e. Escherichia coli, Klebsiella pneumonia and Staphylococcus aureus by using disc diffusion method Acute oral toxicity study on mice had been done and throughout the two weeks of the treatment no significant changes had been observed in behavioral pattern, clinical sign and body weight of the animals. All data indicates that the formulation could be used as bioactive controlled drug delivery systems. Download
    • Development and Validation of HPTLC Densitometric Method for Estimation of Famciclovir in Plasma

      A rapid and reproducible HPTLC Densitometric Method has been developed for estimation of famciclovir in plasma. Chromatography was performed on aluminium plates coated with 200micrometre layers of silica gel, using Butanol: Acetic acid: Water (7:2:1) as the mobile phase. Densitometric scanning was performed at 254nm with Camag TLC Scanner III. Linear least-square regression analysis showed there is a good liner relationship between peak area and concentration. The method was found sufficiently accurate and precise for pharmacokinetic studies of the drug. Download
    • Development and Evaluation of Extended Release Microspheres of Cyclobenzaprine Hydrochloride using Eudragit and Ethyl Cellulose

      Extended release microspheres of Cyclobenzaprine hydrochloride, a skeletal muscle relaxant which relieves muscle spasm of local origin without interfering with muscle. Cyclobenzaprine hydrochloride is highly water soluble drug, having low oral bioavailability (33-55%) due to extensive metabolism of drug. And the dosage forms available in market were having trice daily administration. The main objective of present study was developed to improve oral bioavailability, reduce the frequency of drug administration, and improve patient compliance. In this study, extended release microspheres of Cyclobenzaprine hydrochloride was prepared by solvent evaporation techniques using Eudragit RS 100, Eudragit RS&RL 100 and Ethyl cellulose as polymers and yield, particle size, encapsulation efficiencies and in vitro release of the prepared microspheres were evaluated. The results showed that percentage yields were influenced mainly by polymer concentration, type of polymer. The encapsulation efficiencies were desired for all the formulations of microspheres developed. Particle sizes of the microspheres were influenced by the concentration of Polymer, type of polymer and stirring speed. From the results of the in vitro study shows that the desired release rate is achieved by CBRS 4, CBRL 3 and CBEC 4 formulations are releasing the drug up to 12 hrs. Download
    • Development and Validation of HPLC Method for the Simultaneous Estimation of Levosulpiride and Esomeprazole in their Combined Pharmaceutical Dosage Formulation

      A simple, efficient, and reproducible HPLC method for the simultaneous estimation of Levosulpiride (LEVO) and Esomeprazole magnesium trihydrate (ESO) in their combined pharmaceutical formulations has been developed and validated. The separation was carried out on HYPERSIL ODS (C18), 250 x 4.6 mm (5 µm) column using Methanol: Water: Trietylamine in the ratio (50:50:0.1, v/v/v) as mobile phase. The flow rate was 1 ml/min and effluent was detected at 224 nm. The retention time of LEVO and ESO were 2.51min and 4.52 min. respectively. The linear range was 15-75 μg/ml and 8-40 μg/ml for LEVO and ESO, respectively. Percentage recoveries for LEVO and ESO were 98.30 - 100.6% and 99.5 - 101.25% respectively. All the analytical validation parameters were determined and found in the limit as per ICH guidelines, which indicates the validity of the method. The developed method is also found to be precise and robust for the simultaneous determination of LEVO and ESO in their pharmaceutical formulation. Download
    • Development and Validation of Spectrophotometric Method For The Determination of Cefadroxile And Cefuroxime Sodium In Pharmaceutical Formulations Via Derivitization With 8-Hydroxy-1,3,6-Pyrenesulfonic Acid Trisodium

      A simple, accurate and precise spectrophotometric method has been proposed for the determination of two cephalosporins, namely; cefadroxile (cefa) and cefuroxime sodium (cefu) in pharmaceutical formulations. The proposed method is based on the derivatization of cephalosporins with 1-hydroxy-3, 6, 8-pyrenetrisulfonic acid trisodium salt, (HPTS). Effects of pH, temperature, standing time and HPTS concentration on the determination of cefi, ceph and cefo, have been examined. Beer’s law is obeyed over the concentration of 1-6, and 0.5-3 μg/mL for cefa and cefu respectively. The detection limits were found to be 0.36 and 0.14 μg/ mL for cefa and cefu respectively,and recovery in range form 95.94-99.53 and 95.27-96.06 for cefa and cefu respectively. This method is simple and can be used for the determination of cefa and cefu in pharmaceutical formulations. Download
    • Development and Characterization of Emulgels For Treatment of Chronic Wounds

      This study was conducted to develop an emulgel formulation for wound healing using Carbopol 940 as the gelling agent and isopropyl myristate as the oily phase. The prepared emulgels were evaluated regarding their rheological behavior and zeta potential. Accelerated stability analyses are a common approach for predicting the long-term stability of pharmaceutical formulations. Five formulations differing only in the proportions of the gel and emulsion were stored for 90 days under two different temperature-controlled conditions (-5 ºC ± 2 ºC/ 45 ºC ± 2 ºC). Zeta potential and rheology measurements indicated that formulations F2 and F3 showed better characteristics for use in treating chronic wounds. Stability analyses showed that the rheological behavior and zeta potential of all of the prepared emulgels remained unchanged during storage for 90 days. As a general conclusion, the results indicated that the emulgel formulations were successful concerning all of the parameters evaluated for wound healing. Download