• Development and Validation RP-HPLC Method for Simultaneous Estimation of Telmisartan and Nifedipine In Synthetic Mixture

      A simple, specific and accurate Reverse Phase High Performance Liquid Chromatography Method was developed for the simultaneous determination of Telmisartan and Nifedipine in Synthetic Mixture. The using Phenomenex Luna C18 (250 mm x 4.6 mm, 5 μm) column in Isocratic mode, with Mobile Phase containing ACN: Water: Methanol in the ratio of (10:20:70 v/v/v) pH 3.8 adjusted with Orthophosphoric acid at detection wavelength 234 nm with flow rate is 1 ml/min and run time is 15 min. the average retention time was found to b 2.563 min and 4.403 min for TEL and NIFE respectively. The calibration was linear in concentration range of 4-20 𝜇g/mL for TEL and 2-10 𝜇g/mL for NIFE. The low RSD (< 2%) Value indicates that the method is precise. The recoveries for TEL and NIFE were found to be in the range of 99-100%. The proposed method was Validated and successfully applied for the estimation of Telmisartan and Nifedipine in Synthetic Mixture. Download
    • Synthesis, Cytotoxicity and DNA Binding of Novel Binuclear Antitumor Complexes Formed by Linking Two "2,2'- Bipyridine Palladium (II)" Moieties Via Alkylene Bisdithiocarbamates

      Five novel dithiocarbamate- bridged binuclear palladium (II) complexes [(bpy) Pd (µ-al-bis-dtc) Pd (bpy)](NO3)2 (where alkylenebisdithiocarbamate, al-bis-dtc=ethylenebisdithiocarbamate (en-bis-dtc, 1); propylenebisdithiocarbamate (pn-bis-dtc, 2); butylenebisdithiocarbamate (bu-bis-dtc, 3); hexylenebisdithiocarbamate (he-bis-dtc, 4) and octylenebisdithiocarbamate (oc-bis-dtc, 5) were synthesized with 2,2'-bipyridine (bpy) as capping ligand (Chart 1). They have been characterized by elemental analysis, UV-vis, FT-IR and 1H NMR spectroscopies and molar conductance. The results of elemental analysis and conductivity measurements confirmed the stoichiometry of ligands and their complexes while the characteristic absorption bands, resonance peaks in NMR and IR spectra confirmed the formation of ligand frameworks around the palladium ions. The biological activity of these palladium complexes was evaluated on chronic myelogenous leukemia cell line, K562, at micromolar concentration, and their targets seems to be DNA of the cell. The 50% cytotoxic concentration (Cc50) values of complex 2 is lower than cisplatin whereas other complexes show Cc50 values higher than cisplatin. The interaction of these complexes with highly polymerized calf thymus DNA (CT-DNA) was extensively studied by means of absorption spectroscopy, fluorescence titration spectra, ethidium bromide (EB) displacement and gel chromatography techniques. All of these water soluble complexes bound cooperatively and intercalatively to the CT-DNA at very low concentration. However, at higher concentration, they denature the DNA. Complexes 1 and 4 break the CT-DNA into two fractions and bound with both of them. Download