• In-Vitro Antilithiatic Effect of Ethanolic Extract of Codiaeum Variegatum (L.) Blume

      Objective: The aim of the study is to investigate the antilithiatic activity of ethanolic extract of Codiaeum Variegatum (L.) Blume by in-vitro method. Methods: The ethanolic extract was evaluated for its antilithiatic activity by preparing synthetic urine. The crystal size development was monitored in sample drops every five minutes by polarized microscope. The experiments were carried in absence of plant extract and in presence of 25, 50, 75, and 100% of ethanolic extracts. Result: In the presence of plants extracts, the length and the width of the crystals were reduced. The average length of the crystals grown in the presence of the inhibitors was less than that of the control sample. Conclusion: In-vitro study shows that the ethanolic extract posses antilithiatic activity and further in vivo study is required to confirm its mechanism of action. Download
    • Stability Indicating RP-HPLC Method Development and Validation for the Determination of Potential Degradation Impurities of Efavirenz, Emtricitabine and Tenofovir in Combined Pharmaceutical Dosage Form

      A simple, very accurate and precise stability indicating RP-HPLC gradient was developed to determine related substances of Efavirenz, Emtricitabine and Tenofovir disoproxil from tablet dosage form. The experiment was carried out on Zorbax SB-Phenyl, (250 mm X 4.6 mm), 5 µm column using the gradient composition of buffer pH 3.7 as mobile phase A and mixture of methanol, acetonitrile and tetrahydrofuran (50: 50: 2 v/v/v), degas as mobile phase B at flow rate 1.2ml/min and detection wavelength 265 nm The new proposed method was simple, accurate, precise, linear and rugged. Method was validated as per ICH guidelines1, 2, 3, 4 for simultaneous determination related substances of Efavirenz, Emtricitabine and Tenofovir disoproxil in tablet dosage form hence can be used for routine analysis. Download
    • Isolation and Evaluation of Mucilage from Linum Usitatissimum Seeds

      The aim of the current research work is to isolate the mucilage from Linum usitatissimum and test it for different phytochemicals, characteristic functional groups, pH, swelling index and viscosity. It was found that mucilage contains carbohydrates, polysaccharides, mucilage, gum and protein which are common composition of polymers obtained from the natural sources. Loss on drying, ash value, acid soluble ash, water soluble ash values are 5.2%, 6.3%, 5.78%, 4.98% respectively. pH of the extract is 7.1 and swelling index is 8.5, viscosity at different speed is found in the range of 20.65-40.7 centipoise. It can be concluded that isolated polymer is biocompatible, method of isolation is relatively simple and suitable for use as pharmaceutical excipient. Download