• Qualitative Assessment and Investigation of Antifungal Activity of Methanolic and Petroleum Ether Extracts of Tectona Grandis Linn Seeds

      Tectona grandis has been claimed to be useful in treatment of microbial and fungal infections and the current literature survey revealed that no systematic approach has been made towards documentation of this claim. The currently available synthetic antimicrobial agents are associated with numerous serious side effects like hypersensitivity, crystalluria, photosensitivity, hepatic and renal damage, ototoxicity, neurotoxicity etc. These adverse effects either restrict the continuation of therapy or demand for additional drug/s to combat them. This in turn indicates the need of alternative value addition therapy. In light of this, Methanolic and Petroleum ether extract of Tectona grandis was screened for its antifungal activity by using fungal culture but none of extracts were found to be significant in this regard. The claim mentioned in an ancient period may be of value especially in absence of today’s effective antifungal agents. As far as today’s minimum standard required, it cannot be promoted as antifungal as per these observation. Download
    • Development and Validation of UV Spectrophotometric Method and RP – HPLC Method for Simultaneous Estimation of Teneligliptin and Pioglitazone In Synthetic Mixture

      The present Article portrays simple, sensitive, accurate, precise and cost effective First order derivative Spectrophotometric method and RP-HPLC method for the simultaneous estimation of Teneligliptin and Pioglitazone in Synthetic Mixture. In The first order derivative method absorption at 228.5 nm (zero crossing point for Pioglitazone) was used for Teneligliptin and 269.2 nm (zero crossing point for Teneligliptin) was used for Pioglitazone. The linearity was taken in the concentration range of 2-10 µg/ml for Teneligliptin and 3-15 µg/ml for Pioglitazone with correlation coefficient (R2) 0.995 and 0.997, respectively. For The RP-HPLC method linearity was taken in the concentration range of 1- 5 µg/ml for Teneligliptin and 1.5-7.5 µg/ml for Pioglitazone with correlation coefficient (R2) 0.998 and 0.996, respectively. Proposed technique has been validated as per ICH guideline and successfully applied to the simultaneous estimation of Teneligliptin and Pioglitazone in their Synthetic Mixture. The results of analysis have been validated statistically and by recovery studies. Download
    • Qualitative Assessment and Investigation of Antifungal Activity of Methanolic and Petroleum Ether Extracts of Tectona Grandis Linn Seeds

      Tectona grandis has been claimed to be useful in treatment of microbial and fungal infections and the current literature survey revealed that no systematic approach has been made towards documentation of this claim. The currently available synthetic antimicrobial agents are associated with numerous serious side effects like hypersensitivity, crystalluria, photosensitivity, hepatic and renal damage, ototoxicity, neurotoxicity etc. These adverse effects either restrict the continuation of therapy or demand for additional drug/s to combat them. This in turn indicates the need of alternative value addition therapy. In light of this, Methanolic and Petroleum ether extract of Tectona grandis was screened for its antifungal activity by using fungal culture but none of extracts were found to be significant in this regard. The claim mentioned in an ancient period may be of value especially in absence of today’s effective antifungal agents. As far as today’s minimum standard required, it cannot be promoted as antifungal as per these observation. Download
    • Development and Validation of UV Spectrophotometric Method and RP – HPLC Method for Simultaneous Estimation of Teneligliptin and Pioglitazone In Synthetic Mixture

      The present Article portrays simple, sensitive, accurate, precise and cost effective First order derivative Spectrophotometric method and RP-HPLC method for the simultaneous estimation of Teneligliptin and Pioglitazone in Synthetic Mixture. In The first order derivative method absorption at 228.5 nm (zero crossing point for Pioglitazone) was used for Teneligliptin and 269.2 nm (zero crossing point for Teneligliptin) was used for Pioglitazone. The linearity was taken in the concentration range of 2-10 µg/ml for Teneligliptin and 3-15 µg/ml for Pioglitazone with correlation coefficient (R2) 0.995 and 0.997, respectively. For The RP-HPLC method linearity was taken in the concentration range of 1- 5 µg/ml for Teneligliptin and 1.5-7.5 µg/ml for Pioglitazone with correlation coefficient (R2) 0.998 and 0.996, respectively. Proposed technique has been validated as per ICH guideline and successfully applied to the simultaneous estimation of Teneligliptin and Pioglitazone in their Synthetic Mixture. The results of analysis have been validated statistically and by recovery studies. Download
    • Monoclonal Antibodies Present New Opportunities in Disease Diagnosis and Treatment

      The production of MAbs by hybridoma technol¬ogy was discovered in 1975 by Georges Kohler of West Germany and Cesar Milstein of Argentina. Who developed a technique to fuse splenocyte cells (separated from the spleen of an immunized mouse) with tumorous myeloma cells., our vision for antibodies as tools for research for prevention, detection and treatment of diseases, vaccine production, antigenic characterization of pathogens and in the study of genetic regulation of immune responses and disease susceptibility has been revolutionized. Recently, MAbs have been widely applied in the field of clinical medicine. They are also used in the tissue typing, enzyme linked immunosorbent assay, radio immunoassay, immunological intervention with passive antibody, or magic bullet therapy with cytotoxic agents coupled with anti-mouse specific antibody. Download
    • Mucoadhesive Microspheres of Anti-allergic Agent for Nasal Delivery

      The objective of research work was to develop and optimize mucoadhesive microspheres of anti-allergic agent (cetirizine) for nasal delivery with the aim to enhance the residence time and improve therapeutic efficacy and at the same time increase the local absorption of drug and reducing systemic side effects and also to develop unique delivery system for patients suffering from allergy and rhinitis. Mucoadhesive microspheres (Chitosan based) of cetirizine were prepared by emulsification-crosslinking method in different ratio. Glutaraldehyde was used as crosslinking agent. The mean particle size was significantly increased when high concentration of chitosan was used. Aqueous to oil phase ratio, stirring rate and dioctyl sodium sulfosuccinate (DOSS) concentration also influenced the particle size distribution of the microspheres. Microspheres were evaluated with respect to the production yield, particle size, entrapment efficiency, swelling index, FT-IR, in vitro mucoadhesion, cumulative percentage drug release, histological study and stability studies. Formulation Cf3 was found to be optimized. The optimized formulation Cf3 was mucoadhesive in nature which adhere onto the mucus and increase the residence time within the nasal cavity. Download
    • Study of Liver Function Test in Tuberculosis Treatment

      Tuberculosis is major problem in India. In India about 1.96 millions of the new cases were reported annually. About 3.8 million of the new cases showed prevalence of the tuberculosis. Antituberculosis treatment is the common cause of drug induced liver injuries or hepatotoxicity in the patients. It has been proved in many studies that the effect occure due the elevated level of the liver enzymes like ALT, AST, ALP. The study was conducted on the patients of DOTs centres in the North India. About 50 patients were selected. The blood samples were collected from the patients and the liver markes were studied before and after drug therapy. The levels of the Alkaline phosphatase, Aspartame transaminase, Bilirubin & Plasma albumin were increased significantly after the drug administration. The study concludes that side effects in the patients with anti-tuberculosis drugs shall be monitored regularly. Download
    • Molecular Imprinting: An Emerging Technology

      This article gives the recent developments in molecular imprinting for proteins. Currently bio-macromolecules such as antibodies and enzymes are mainly employed for protein recognition purposes. However, such bio-macromolecules are sometimes difficult to find and/or produce, therefore, receptor-like synthetic materials such as protein-imprinted polymers have been intensively studied as substitutes for natural receptors. Recent advances in protein imprinting shown here demonstrate the possibility of this technique as a future technology of protein recognition. This review summarizes the previous and current literature regarding the analytical tools employed for characterization of synthesized MIPs. It is our expectation that this will facilitate researchers to plan their own sophisticated analytical pathway for characterization of MIPs in a more logical and structured fashion, and to begin to appreciate the limitations of the present approaches in this molecularly complex area. Download