• Preformulation Study of Raloxifene Hydrochloride for Transdermal Drug Delivery

      The aim of the present work was to perform the preformulation studies of Raloxifene hydrochloride to determine certain key physicochemical parameters which can be further used to develop a feasible transdermal formulation. Preformulation testing is done to generate data useful to the formulator in developing stable and bioavailable dosage form. On oral administration RLH undergoes extensive first pass metabolism resulting in poor bioavailability so the target was to prepare a transdermal drug delivery system (patch) of RLH to increase its bioavailability. The drive of formulating a patch is to provide a safe, simple and alternative route of administration for the prevention of osteoporosis in post-menopausal women. Development of analytical method, solubility profile, partition coefficient, particle size and drug excipient interaction were investigated as per the requirements of preformulation study. All the results found testify the candidature of RLH for transdermal drug delivery. Download
    • Formulation and Evaluation of Topical Spray Containing Anti Acne Agent

      Objective: The objective of the present study was to formulate a novel topical spray containing Antiacne agent. Comprising drug and other non-toxic excipients (mixture of propane and butane) LPG as propellant. Study was designed to increase the absorption of drug from the human skin. Therefore clear clinical need exists for development of suitable formulation of drug that has improved permeability and absorption, with the potential transparent thin film with the once daily dosing. An optimum formulation was to be study for skin irritation study, in-vivo drug release and finally for stability study. Experimental work: The preformulation studies for drug was carried out and compatibility of drug in formulation with different excipients was checked. Solutions for topical sprays were filled in Aluminium containers fitted with continuous spray valve. Primary screening of variables like polymers, penetration enhancers and solvents were done by preparing trial batches. Final batches were prepared comprising of polymer Eudragit E100 (flim forming polymer), penetration enhancers such as IPM and PG act as plasticizer, solvent and co-solvent such as Iso Propyl alcohol and ethanol respectively. Prepared Tazarotene topical spray formulations were evaluated for different parameters. Tazarotene topical spray included determination of delivery rate, delivery amount, pressure test, drug content, minimum fill, leakage test, flammability, spray patterns, particles size, etc and as well as In-vivo Skin irritation study and In-vitro drug released, Finally, optimised formulation Was kept for stability study as per ICH guidelines. Results and discussion: Absence of physical and chemical incompatibility during compatibility study revealed that Tazarotene is compatible with container closure and excipients. Tazarotene topical spray formulation T6 was found to be the best formulation Evaluation data of different formulation for Physico-chemical test, performance test and ex-vivo diffusion studies indicated the effectiveness of IPM as penetration enhancer. T6 was further proved non irritant and stability study in accordance with ICH guidelines indicated that the optimised formulation was stable. Conclusions: A novel type of formulation comprised of Tazarotene, Eudragit E100, IPM and organic volatile and non-volatile solvents, was used to develop a new topical spray formulation. This novel topical spray formulation was transparent solution with good, early evaporation and ease of application. In addition the research results showed the resultant invisible thin film with excellent carrier and permeability enhanced effect of IPM on drug. Finally, skin irritation test proved that the spray formulation was safe to be used for topical delivery. In summary, the novel formulation of Tazarotene, Eudragit E100, IPM and other excipients may provide alternate dosage form to Tazarotene gel formulation. Download
    • Field Flow Fractionation

      Field flow fractionation (FFF) is a separation technique conceived by J. Calvin Giddings. FFF is a separation technique where a field is applied to a solution which is pumped through a long narrow channel which is perpendicular to the direction of flow in order to cause separation of particles present in the fluid, dependent on their differing mobility’s under the force exerted by field. Analytes can be separated by different mechanisms. The mode of operation determines the elution order of analytes, along with other separation characteristics such as selectivity and resolution. Three widely used modes that can be implemented in any FFF technique are normal, steric and hyperlayer modes It uses most of the ancillary equipment employed in chromatography such as injector valves, pumps for the carrier liquid delivery, detectors, and some data acquisition devices such as chart recorders or more conveniently computers. It is particularly suitable for macro-molecules, colloidal and particulate materials extending from a few hundred to 10 Da. Download