Critical Micelle Concentration is a surfactant concentration above which micelles form. Micelle formation favors solubilization of poorly soluble substances in a required middle. Papain is a proteolytic enzyme used in wound care. It is partially soluble in water and slightly stable in formulations. Hydrogel has been prepared with papain and polysorbate 80 as a solubilizing agent serially, with and without L-cysteine. This study aimed to determine the critical micelle concentration (CMC) by measuring surface tension (Du Nouy) and thermodynamic stability using zeta potential technique. The use of L-cysteine caused a decrease in CMC, and polysorbate 80 almost achieved a zero zeta potential. Nevertheless, in both serials, papain hydrogels were homogeneous and thermodynamically stable.
Nicoletti, C.D.*; Ferreira, V. F.; Cruz, R.C. ; Futuro, D.O.